2.1 Input Requirements

The following information is required before starting the INDUCE-seq Analysis workflow. Confirm you have access to all items before proceeding.

Input	Description	Required?
Sample sheet	The sample_sheet.csv used during the sequencing run for your experiment.	Required
Sequencing data (FASTQs)	The *.fastq files produced by the sequencing run.	Required
Nuclease information	Type and characteristics of the nuclease used for editing, including the guide sequence. If known, cut site positions relative to the guide sequence can be supplied for more accurate homology-based analysis.	Required
Sample lab metrics	Post-library preparation QC metrics: amount of gDNA extracted during library preparation (ng), amount of library per sample (ng), and amount of sequencing-ready library (pM).	Optional — enables QC based on laboratory inputs

Note

For guidance on obtaining sample lab metrics, refer to the On-Demand INDUCE-seq Solution Assay User Guide.