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9.9 Breaksite Plots

Breaksite plots show the distribution and frequency of breaks at per-base resolution for each non-singleton nominated break site. Visualisations are organised into subfolders per condition.

How to read a breaksite plot

  • Red bars — breaks on the positive (+) strand.
  • Blue bars — breaks on the negative (–) strand.
  • For edited samples, the breaks observed in the appropriate control at the equivalent location are shown in a lower panel.
  • The edited and control panels share the same y-axis scale to enable direct comparison.
  • If a default or user-defined nuclease was specified, defined sense and antisense break positions are overlaid as dashed lines.
  • Hover over a stack of breaks to view position, count, strand, and distance from PAM.

Breaksite plot showing per-base strand-resolved breaks (red = plus strand, blue = minus strand) for treated and control samples

Breaksite plot with hover tooltip showing position, count, strand, and distance from PAM start